December 2013 Edition

IJASR Volume 1- Issue 1, December 2013 Edition 

All listed papers are published after full consent of respective author or co-author(s).
For any discussion on research subject or research matter, the reader should directly contact to undersigned authors.

Comparative karyological studies on the two Egyptian schistosome vectors, Biomphalaria glabrata and B. alexandrina, with reference to chromosomal aberrations due to Za’ater plant

Ahmed A. Abdel-Haleem

Abstract: – Karyological character variations, included G-banded patterns, in the two Egyptian vectors of schistosomiasis, Biomphalaria glabrata and B. alexandrina (Gastropoda, Planorbidae) had studied. Chromosomes of the present snails that treated with Za’ater-plant extract (Origanum syriacum, Labiatae) produced three types of chromosomal aberrations; chromosomal addition in the two species, chromosome gab in B. glabrata and chromatid break in B. alexandrina. The results had been analyzed using suitable statistical methods. The present study estimated the effective dose of Za’ater that led to revealing these chromosomal aberration-types.

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Electrochemical analysis of response behavior of silver selective electrode based on triazine derivatives

W. H. Mahmoud, Gaber A. W. El-Inany, F. M. E. Omar and R. M. El-Tohamy

Abstract: – The study concerned with the development and construction of three potentiometric sensors selective for Ag+ depends on incorporation of some insecticides compounds derivatives of triazine; such as [Diniconazole (DC) (I), Ametryn (AM) (II), Prometryn( PM) (III)] in the membrane and forming relative complexes by conditioning these sensors in Ag+ solution. These sensors showed very good sensitivity and selectivity toward Ag (I) with good Nernstian slopes 56.7±0.2, 48.7±0.1, 55.0±0.8 mV/decade with lower limit of detections  5.75.0X10-6,  5.3.0X10-6, 3.5.0X10-6 M in pH range 3.5-7, 4.5-8, 4-8 over the concentration range 10-5 -10-2 M respectively, all the factors which affected on the sensitivity and selectivity of these sensors were studied, such as the percentage of ionophore in the membrane, the effect of solvent mediator, the concentration of internal solution, PVC derivatives and pH media. The comparison between the three sensors are evaluated, from the results obtained, it was found that the more selective one is the sensor (I) which has the Nernstian slope 56.7 mV/decade, the lowest detection limits 5.75×10-6 M and fast response time less than 10 sec. All the proposed sensors are used for detection and determination of silver ion in its solution by direct potentiometric measurement technique with 0.1 M NaCl and gave a sharp inflection points.

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Histological Study of the Effect of Cisplatin on the Liver of Adult Male Albino Rat

Hesham, A. Ahmed and Mohamed M. Ghobara

 Abstract: – Objective: This study was designed to assess the toxic effect of Cisplatin drug on the histological structure of the liver of adult male rats and the degree of improvement after abstinence of the drug for 2 months for recovery as well as its effect on the  body weight. Results: The body weights of cisplatin treated rats were significantly decreased in comparison to controls (P<0.001) Two months after withdrawal of the drug, there was a significant increase in body weights of the rats in the recovery group (P<0.01). Both light and electron microscopic studies revealed that low doses of cisplatin caused hepatotoxicity manifested by cytoplasmic vacuolization of hepatocytes, congested blood sinusoids, apparent increase in the number of Kupffer cells and focal mononuclear cellular infiltration. However, some specimens showed distortion of hepatocytes with rarified cytoplasm and focal necrosis while others revealed severely congested blood sinusoids. At ultrastructural level, hepatocytes showed cytoplasmic vacuoles (dilated cisternae of endoplasmic reticulum), irregular nuclei and many small mitochondria with ill distinct cristea. In some cells, the observed cytoplasmic vacuoles were remarkably large. Apparent increase in the number of Kupffer cells with many secondary lysosomes and lipofuscin deposits were noticed. Two months after withdrawal of the drug, lesser and smaller cytoplasmic vacuoles in the hepatocytes were observed, while others appeared more or less normal

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Electrochemical analysis to construct specific sensor, selective for mercury (II) ions 

W. H. Mahmoud, Gaber A. W. El-Inany, F. M. E. Omar and R. M. El-Tohamy

Abstract: – The present work deals with preparation and characterization of mercury selective membrane-sensor based on prometryn compound as ionophore. This sensor exhibited good response to mercury (II) ion over the concentration range 10-5-10-2 M with Nernstian slope 27.7±0.l mV/decade at pH 3 with response time less than 20 sec. and the lower limit of detection 6×10-6 M. The response characteristics of this sensor are studied. The comparison between the proposed sensor and the other sensors which are reported in the literature is noted.

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Physiological effect of two heavy metals on esterase isozyme of digestive gland of garden flatworm Bipalium kewense (Moseley, 1878), 1ST record in Egypt

Abdel-Haleem, A. A. &, Beltagi, S. M. and Ali, A.S.

Abstract: – Taxonomically, electrophoresis of isozymes might possibly contribute a better tool for taxonomy within the genera and species. However, physiological studies on the Egyptian flatworms are insufficient. Generally, esterase isozymes are found in some organs of flatworms, including digestive gland. Thereby, the present work included electrophoretic feature of esterase isozymes in the garden worm Bipalium kewense, 1st record in Egypt, as well as studying the effects of two heavy metals, zinc and copper, on esterase isozymes of digestive gland of this species. After the electrophoretic run, the Bipalium-esterase had been classified into three groups; the fastest arylesterase, aliesterase, and the slowest cholinesterase. The present results revealed that the most sensitive components to Zn and Cu, for 72 hours, were aliesterase and cholinesterase whereas arylesterase group showed the highest susceptibility to both metals.

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Functional biochemistry and microbiology of human Saliva  Review Article

M.Abdelrazik, and Khalil H. Alali

Abstract: – Saliva, a thick, colorless, fluid that is constantly present in the humans mouth. It is composed of water, mucus, proteins, mineral salts, and amylase. As saliva circulates in the mouth cavity it picks up food debris, bacterial cells, and white blood cells. Three major pairs of salivary glands and many smaller glands scattered in the surface tissue of the cheeks, lips, tongue, and palate contribute to the total amount of saliva. Small amounts of saliva are continually being secreted into the mouth, but the presence of food, or even the mere smell or thought of it, will rapidly increase saliva flow.

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